Coding

Part:BBa_K2488000:Design

Designed by: Anastasiya Oguienko   Group: iGEM17_Moscow_RF   (2017-10-27)


Phytase of Obesumbacterium proteus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 319


Design Notes

Unfortunately, our gene is integrated into the plasmid between restriction sites of SpeI and PstI, but we hope that it would not prevent everyone from using it in ones work.


Source

Genomic DNA of Obesumbacterium proteus, but our team got it from our PI, who provided us a plasmid pUV3-Op, containing the gene of phytase

References